Beckman Coulter Life Sciences, a supplier of laboratory automation and innovation and an working firm of Danaher Company, has launched the CytoFLEX mosaic Spectral Detection Module, the business’s first modular spectral circulate cytometry answer, the corporate introduced on March 18, 2025 (1).
The CytoFLEX mosaic Spectral Detection Module provides considerably higher fluorescence sensitivity when linked to the CytoFLEX LX or S Movement Cytometers. The answer addresses challenges with dim and complicated multicolor experiments as a result of it could detect nanoparticles as small as 80 nm. The instrument gives two unmixing algorithms and as much as 10 autofluorescence channels, with as much as 88 detection channels. Consequently, richer knowledge will be generated from spectral evaluation that may assist untangle the complexities of organic methods and ailments quicker. It additionally permits for a nuanced understanding of immune cell subsets and their features and interactions by concurrently detecting a number of markers.
“As a central characteristic of the CytoFLEX platform, this innovation is concentrated on pairing ease-of-use with highly effective, delicate acquisition know-how to take away the intimidation encountered in lots of circulate cytometry devices,” mentioned Matthew Goff, product supervisor, Beckman Coulter Life Sciences, in an organization press launch. “Investigators can now simply change between their true typical detection system and this superior spectral detection system, whereas sustaining a well-known pattern administration and consumer expertise, all with out having to spend money on a separate instrument. Together with experimental continuity and evolution supplied by a extra versatile method, this delivers a singular answer for sustainability wanted by many establishments.”
In comparison with typical circulate cytometry (2), spectral circulate cytometry acquires the complete emission spectrum of every antibody-associated fluorophore throughout all lasers, slightly than simply measuring the height of emission. This superior means delivers a extra detailed and exact characterization of mobile properties, which might then advance the understanding of advanced organic methods and ailments inside every pattern in comparison with older, conventional approaches, based on the corporate within the launch.
The CytoFLEX mosaic spectral detection module runs on a singular algorithm that’s able to bettering decision in comparison with different out there strategies. It additionally provides unmixing accuracy checks for making certain effectivity. Accompanying the instrument is the CytExpert software program, utilized in spectral cytometry to cut back the necessity for intensive coaching, which might thus velocity up the transition to spectral know-how. The module additionally comes with instruments to ease the complete spectral workflow and provides entry to the FluoroFinder panel builder and a Cytobank premium license that present all of the instruments wanted to transition from pattern preparation to evaluation of advanced spectral knowledge in a simple method.
“I discovered the software program very straightforward to be taught and was in a position to begin my first experiments in just a few hours,” mentioned Tamar Tak, coordinator of the Movement Cytometry Facility at Leiden College Medical Middle, within the press launch. “A characteristic I actually like is that it permits me to do an intensive QC [quality control] of the unmixing. You get a plot with all of the normalized spectra of your fluorochromes and a desk with similarity indices. This permits me to see if a tandem dye has degraded, or maybe the mistaken antibody was added. The autofluorescence settings are one other spotlight, permitting flexibility relying in your samples—from human tissue to advanced samples of microorganisms.”
The brand new module is on the market in two configurations, the CytoFLEX mosaic 88 for the CytoFLEX LX Movement Cytometer and the CytoFLEX mosaic 63 for the four-laser CytoFLEX S Movement Cytometer.
References
1. Beckman Coulter Life Sciences. Beckman Coulter Life Sciences Launches Trade-First Modular Spectral Movement Cytometry Answer. Press Launch. March 18, 2025.
2. McKinnon, Ok. M. Movement Cytometry: An Overview. Curr. Protoc. Immunol. 2018, 120, 5.1.1–5.1.11. DOI: 10.1002/cpim.40
